Inventory

–WIP–

As I build up lab capacity, there are things one has to buy in bulk which can be easily shared. I’ve already dumped a load of extras on one friend, and after the first PDX diybio meetup I’ve decided I should write up what I have (and what gaps remain) so that others can plan around it! If you have projects that could benefit from some of this, do let me know - together we can do more :) I’m throwing things in as I think of them, organization etc subject to change.

Tools

  • 3D Printer (Ender 3 V3 SE, cheap and outdated but works great) - happy to rattle off PLA prints as needed
  • Microscope (XXX)
  • Micropipettes (2-10ul, 10-100, 20-200)
  • Hotplate
  • Janky DIY stirplate
  • Rotary tool that doubles as a 1.5ml centrifuge
  • Blowtorch, Camping Stove
  • Still Air Box (happy to lend out)
  • Assorted hand tools
  • Jig Saw, Drill, Impact Driver
  • Thermal Camera
  • Soldering iron (+ a portable one)
  • Inflatable SUP (borrowable)

Consumables

Reagents

Genes/Strains

(Older inventory to start from)

Electronics & Soldering Station Soldering Gear:

Soldering Iron Station (blue handle) Pinecil Mini Soldering Iron Solder wire spool Heat shrink tubing Solder fume extractor (fan unit) “Helping hands” tool with alligator clips Components & Microcontrollers:

Raspberry Pi / Picos / ESP chips (mentioned in audio/labels) Component storage drawers (Labeled: RGB LEDs, UV LEDs, IR LEDs, Displays, Capacitors, DC Motors, Servos, Potentiometers, Switches, LDR) Breadboards and jumper wires Breakout boards/ribbon cables Resistor kit Test Equipment:

Digital Multimeter (Red/Black, appears to be manual range) Thermal Camera (handheld) Power:

Anker Solix Portable Power Station Corsair RM1000x PC Power Supply (adapted for bench use) Variable DC Power Supply adapter board (breakout for the PSU) Various USB cables and wall adapters (12V w/ barrel jack, 5V) Tools & Hardware Power Tools:

Ryobi One+ Jigsaw Ryobi One+ Drill/Driver Ryobi One+ Impact Driver Ryobi Handheld Vacuum (18V) Ryobi Battery Charger Dremel Rotary Tool (on flex shaft hanger/stand) - mini Ryobi one, underpowered Hot Glue Gun (red) Heat Gun / Blow Torch (small handheld butane torch) Blowtorch with food-safe brazing supplies Hair dryer Hot plate (set temp, nice for desoldering but also chemistry) Hand Tools:

Screwdrivers (various, including green handle Snap-on/similar and precision sets) Wire flush cutters (blue handle) Wire strippers Locking pliers (Vise-Grips) Adjustable wrench Hammer Hacksaw Japanese Pull Saw Clamps (Ratchet clamps, spring clamps, not many) Calipers (Vernier plastic ones only) Pen knives / X-Acto knives Scalpel Small tweezers (fine tip) Chemistry & “Wet Lab” Chemicals & Reagents:

Agar Agar Powder Agarose (for gel electrophoresis) Sodium Alginate Calcium Chloride Calcium Carbonate Sodium Citrate Sodium Hydroxide (drain cleaner) Vegetable Glycerin Distilled Water (gallon jugs) Citric Acid Vitamin C Epsom salts KDP Hydrogen Peroxide Ammonium Nitrate (small amount, dry + some dissolved w/ CaCO₃ ppt.) Potassium permanganate powder Ethyl Acetate Pure caffeine Activated Charcoal Stains & Kits:

Zymo DNA Shield Methylene blue Gram stain kit - Crystal Violet, Iodine, Safranin SeeGreen DNA stain Gel buffer solution Luminol kit Cyanotype kit Cooking Science / Modernist Pantry:

Access to basic pantry staples Food coloring pack Solvents & Cleaners:

70% Isopropyl Alcohol in a spray bottle, also 90% Acetone (Klean Strip) Lacquer Thinner Turpentine / Mineral Spirits Everclear (ethanol) WD-40 Dawn Dish Soap Clorox Bleach Spray Wet Ones (wipes) Labware:

Digital Scale (SF-400D) DIY Stir Plate Microscope (White, compound binocular) Microscope slides and cover slips Petri dishes (90mm and 60mm) Beakers (glass and plastic, 100ml-1L, various sizes) Graduated cylinders/plastic cups Pipettes (disposable), Syringes (3ml w/ needles), and a P200 pipette with tips (5-200µL) Syringe filters Falcon tubes (15ml and 50ml) 1.5ml Eppendorf tubes (lots!) 3D printed tube holders 4oz glass jars (8) and 4oz PP tubs with attached lids (for tissue culture) TLC plates pH Test Paper Bibulous paper from gram kit Optics & Light Lasers & Lights: “Normal” Flashlight (Large black handheld) UV Flashlight Green Laser Pointer Diffraction Gratings (slide format) Safety: Laser Safety Goggles (Orange/Red lenses) Adhesives, Paints & Finishing Adhesives: Elmer’s Carpenter’s Wood Glue Mod Podge Super Glue (various tubes) Kwik Seal (Kitchen/Bath adhesive caulk) Hot glue sticks Paints: Krylon Fusion Spray Paint (Blue Hyacinth seen) Rust-Oleum Automotive Primer 3D Printing & Fabrication Printer:

FDM 3D Printer (Ender 3 v3 SE) Raspberry Pi connected to printer Supplies:

PLA Filament (green, white, black) Miscellaneous / Safety VersaShield Nitrile Gloves N95 Face Mask Safety Glasses (clear) Air Purifier (White cylindrical unit on floor) Pegboard organization system Sorting bins/organizers Bio Strains & Plasmids E. coli cultures (BL21 and MM294 strains, various colorful plasmids) Agrobacterium with RUBY plasmid pDawn and pDusk plasmids (light-inducible) Media & Antibiotics LB broth mix Marmite for making ‘marmite broth’ plates/media RIff/Spec agar mix Kanamycin (supply from Seb + fish medicine “fiskKan”) Ampicillin (enough for a few more plates) Usually some agar plates ready-made Plant Tissue Culture Plants in jars starter kit (various growth hormones, MS media) Agrobacterium injection mix (50ml) 4oz PP tubs with attached lids (for tissue culture) Molecular Biology Mini-prep kit (Bioland, silica columns) Agarose + SeeGreen DNA stain + gel buffer (in Chemistry) 9V batteries for gel PS or a fixed 32V PSU Equipment & Consumables A still air box (thanks Dave) A little camping stove (for flaming loops + sterile bubble) A grow pad - used as incubator sometimes 3D printer hotbed - also doubles as incubator A 3D printed microcentrifuge for 1.5ml tubes (uses rotary tool) Crushed ice / hot water bath when needed Remnants of a Carolina Bio transformation kit Supplies Cling wrap Aluminium foil Little PP sauce tubs Ziploc sandwich bags (fit two petri dishes) Activated Charcoal Misc Diatomaceous earth Label maker Piezo microphone, some misc music gear Blood pressure monitor Macro camera with great zoom (Raynox DCR250 on a Lumix FZ80 camera) Kapton tape, Duct tape, Gaffer tape, insulation tape, plumbers tape Random wood screws etc A 6W UVC lamp (for mutagenesis, sterilization, spectroscopy) A piezo lighter spark thingee Compressor for car tires Some old grow lights, hydroponic pH UP and DOWN, and plant-related bits Work gloves Laser + UV safety goggles